Job Summary: Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.
MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).
MDL is looking for a Laboratory Technician. The lab technician is responsible for accessioning and processing specimens, performing automated and manual extractions, Real-Time and Conventional PCR, gel electrophoresis, Pyrosequencing, cell culture, and Next-Generation sequencing.
Essential Functions:Accessioning:
- Print labels and attach to requisitions and the corresponding specimens
- Properly accession multiple specimen types
- Take photographs of all specimens received
- Properly identify any discrepant information
- File all paperwork in order and distribute to the appropriate QC bins
- Properly scan accessioned requisitions
- Scan 2-D barcode elution tubes used for extraction
- Properly locate and record temperatures for all necessary stations
- Perform Phenol/Chloroform manual extractions on biopsies and ticks
- Properly separate samples into their organic and inorganic layers via phenol/chloroform
- Properly perform quantitative analysis and hydrate all ticks and biopsies
- Properly extract all samples types using a provided XG sheet as a template
- Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
- Properly dispose of all waste material when required; both hazardous and non-hazardous
- Attend to robotics to address any user interactions and/or robotic malfunctions
- Manually extract all necessary samples via commercial kits
- Perform manual RNA extractions via Qiagen kit for all necessary samples
- Perform robotic RNA extractions via VX Reagents kit for all necessary samples
- Perform manual and robotic Conventional method PCR amplification
- Properly load and start thermocycler machines
- Perform manual and robotic Real-time method PCR amplification
- Properly load and start Rotor-gene thermocycler
- Perform initial analysis on completed Real-time PCR
- Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
- Properly load and start Bio-Rad CFX thermocycler
- Perform initial analysis on completed Acoustic Real-Time PCR
- Properly identify which completed PCRs from the thermocycler require gel electrophoresis
- Properly identify when to make and/or use 2% versus 2.5% gels
- Complete blank gel template sheets to combine short PCR sheets
- Use dye to stain all necessary samples with the appropriate volume on a test by test basis
- Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
- Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
- Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors
- Properly perform MagPlex PCR
- Properly remove any unused dNTPs and primers within the amplified PCR product with the use of ExoSAP-IT
- Properly perform Single Base, and Allele Specific Extension
- Properly attach samples to appropriate bead mixtures, and subsequently label samples with Strptavidin-R-phycoerythrin (SAPE)
- Properly load and start BioPlex machine
- Properly analyze all run data
- Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
- Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
- Properly analyze growth plate to determine antibiotic susceptibility or resistance
- For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
- Identify and pick colonies for PCR
- Perform CAMP test and analyze for zones of hemolysis
- Utilize the Illumina platform to test for BRCA, Pharmacogenomics, and Cystic Fibrosis
- Properly perform a series of nested PCRs
- Properly attach samples to appropriate bead mixtures
- Properly perform bead cleaning to remove unused dNTP’s and primers within the amplified PCR product
- Properly perform gel purification
- Properly load and operate multiple Life Technologies and Illumina equipment
- Perform Sanger Sequencing
- Properly analyze/export all run data
- Performs other similar or related duties as required or assigned.
- Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
- Assures customer privacy is maintained at all times.
- Participates as a team member and is accountable for own work responsibilities.
- Assures information is delivered in a manner that is supportive, timely and understandable.
- Initiative is demonstrated to proactively diagnose and resolve problems.
- Productive work habits are consistently displayed.
- Accuracy and quality are maintained for all activities.
- 9 month Clinical Laboratory Technician training
- Ability to sit, stand, walk and perform repetitive motion (manual pipette)
- Proficient visual and audio skills
- Laboratory setting
- Use of personal protective equipment
- Bachelor Degree in Biology, Chemistry, or related science field
- Competitive Salary
- Medical, Dental & Vision Insurance
- Short and Long Term Disability
- Life Insurance
- Dependent Care Flex Spending Account
- Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)
- Identity Theft Protection
- 401(k) Plan
- Paid Vacation & Holiday
- Business Casual Environment
Medical Diagnostic Laboratories is a member of Genesis Global Group, an equal opportunity employer.